Back when I was in Japan (look here for more details: https://connectingjapandgermany.wordpress.com/) I had the chance to work for one of the best optic companies in the world. Nikon. I was able to spend two weeks in the life science department and was able to do some research. It was the first time to here somehting like superresolution microscopy. In university you always learn everything about the abbe-criteria and the maxima resolution given by (Wikipedia):
- : minimaler Winkel
- : Wellenlänge der beobachteten Strahlung
- : Öffnungsdurchmesser
With tricky illumination and post-processing you can more-less easily double the resolution. Therefore you’re illuminating the object with a sinusodial pattern varied in phase and space. In the fourier domain of the recorded pictures, You’ll get the equivalent phase distribution, which can be superpositioned to a spectrum whichs OTF has half of the original one. This means, that the PSF of the Microscopes lens halfs just by doing some computation.
„Project: DIY Superresolution Microscope (SIM – Structured Illumination Microscopy)“ weiterlesen