A very cheap method to realize a spatial light modulator one can simply use an off-the-shelf LCD module driven by an Arduino. When removing the backlight and putting the LCD in the optical path, it acts as a very cheap mask. Controlling the device via MATLAB or PYTHON is an easy task to do!
In my work I tried to implement a SERVER<-> Client interarction, where Matlab sends command and the Arduino executes them.
„Arduino IL9341 LCD MATLAB/Python/Serial interaction“ weiterlesen
When characterizing cells or any other microscopic objects it’s sometimes useful to guide them through tiny channels when observing or counting them under the microscope. The term is sometimes called as microfluidics. Therefore many different methods exist to create these devices. One method etches channels in glass or plastic, another additively procedure – sometimes called photolithography – cures a photoresist/resin to get a 3D-like structure after washing the uncured parts away. This usually costs lots of money. Further details here: http://pubs.rsc.org/en/content/articlehtml/2016/lc/c6lc00284f
One part of my research was to improve phase-contrast using a brightfield microscope without adding any special optics like the DIC-prism or phasering in Zernike phase-contrast. Defined phase objects are hard to get. Thinking about a light guide embedded in immersion oil could be one chance to have a phase object, but this extends the field of view (FOV) of a 20x/63x objective which was used in my setup.
Cells are usually amorphous and won’t have defined phase retardation. Therefore I was thinking about a way how I can get a defined phase on the cheap with off the shelf components.
„New and super-cheap method to 3D print micro-structures (microfluidic, phase-objects, etc.)“ weiterlesen
Well.. Demand on imaging small structures is widely increasing. In 3rd world countries diagnosis of parasites like malaria is becoming more and more important. Also judging over the water quality is of great importance. General public has hardly any money to spent for those kind of technologies, so why not using things which are around already? I’m not the first one taking this into account, but want to give some inspirations on how a smartphone microscope could look like.
This one was also thought as a present for someone who has a BLACKBERRY PASSPORT, but the upper case for the smartphone can be exchanged to any other case you find. You could either glue or use the two drills to attach it to the main body.
„DIY Universal framwework for smartphone microscopes (Here: Blackberry Passport)“ weiterlesen
Here you can see the working Z-stage using the Zoom lens of the WIFI-driven digitial still camera
Realizing the Microscope using a Smartphones camera makes removing all lenses necessary, thus one needs to destroy a usually working phone.
Even though it’s open-source it’s really expensive. So why not finding a solution which still uses a smartphone, but only for processing raw data which is coming from a 3rd party device. Looking for a good solution having in mind, that the pixelsize should be as small as possible and accessible by a smartphone I’ve found the Sony QX 10 digital still camera. „Holoscop V3 – Sony Wifi-Cam as lensless Microscope (R.I.P)“ weiterlesen
So next Generation Holoscope is coming. And: It looks promising! Everything’s open-source. The concpet will work as follows:
- Raspberry Pi acts as a server; It handles communication between the Smartphone and the Hardware-stuff which is: Synchronisation of the Camera/Illumination Pattern, Driving the motor and sending the images to the phone
- The Smartphone shows a live-view of the live-stream coming from the Picamera and gives the chance to setup stuff like ISO, Exp-Time, etc. Triggering an acquisition set is also possible; It will also do reconstruction processing as it has – at least in my case – a better CPU/GPU than the PI.
- So far a router is needed as a Peer-to-peer Communication is not implmented yet
- Camera Sensor is the one from the PI-Camera module
„Raspberry Pi + Picamera + LCD + Zoomlens .. Holoskop V4“ weiterlesen